Hotspot

rpl32-trnL-UAG

SSC · 120714-121510 · 797 bp

Back to Neuwiedia

Pi 0.197
MarkerSeek score 93.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
797
variable_sites
124
pi
0.197
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
93.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAGCAGTAAAGGCTTTTTC TCCTAAGAGCAGCGTGTCTA 42.9 50.0 57.3 57.9 870-933 bp 1.0 77.8
rpl32-trnL-UAG_p2 GCAGCAGTAAAGGCTTTTTCT TCCTAAGAGCAGCGTGTCTA 42.9 50.0 57.3 57.9 869-932 bp 1.0 77.8
rpl32-trnL-UAG_p3 AGCAGCAGTAAAGGCTTTTTC AGCAGCGTGTCTACCAATTT 42.9 45.0 57.3 57.8 863-926 bp 1.0 77.3
rpl32-trnL-UAG_p4 GCAGCAGTAAAGGCTTTTTCT AGCAGCGTGTCTACCAATTT 42.9 45.0 57.3 57.8 862-925 bp 1.0 77.3
rpl32-trnL-UAG_p5 GCAGCAGTAAAGGCTTTTTCT CTAAGAGCAGCGTGTCTACC 42.9 55.0 57.3 57.8 867-930 bp 1.0 77.2