Hotspot

ndhF-rpl32

SSC · 119856-120539 · 684 bp

Back to Neuwiedia

Pi 0.0282

MarkerSeek score 75.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 684
variable_sites: 16
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTGGTAGCGGAAAAAGAGG	AGAAAAAGCCTTTACTGCTGC	50.0	42.9	57.5	57.3	777-836 bp	1.0	68.6
ndhF-rpl32_p2	ACTGGTAGCGGAAAAAGAGG	GAAAAAGCCTTTACTGCTGCT	50.0	42.9	57.5	57.3	776-835 bp	1.0	68.6
ndhF-rpl32_p3	CTGGTAGCGGAAAAAGAGGT	AGAAAAAGCCTTTACTGCTGC	50.0	42.9	57.5	57.3	776-835 bp	1.0	68.6
ndhF-rpl32_p4	CTGGTAGCGGAAAAAGAGGT	GAAAAAGCCTTTACTGCTGCT	50.0	42.9	57.5	57.3	775-834 bp	1.0	68.6
ndhF-rpl32_p5	CTGGTAGCGGAAAAAGAGGT	AAAAAGCCTTTACTGCTGCT	50.0	40.0	57.5	56.1	774-833 bp	1.0	66.8