Hotspot

rbcL-accD

LSC · 59570-60711 · 1142 bp

Back to Neuwiedia

Pi 0.0256
MarkerSeek score 74.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1142
variable_sites
29
pi
0.0256
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCAAATGGAGCCCTGAACTA GGTTCTTCACTTCCACTGGT 50.0 50.0 57.9 57.4 1353-1398 bp 1.0 75.9
rbcL-accD_p2 TAGCAAATGGAGCCCTGAAC GGTTCTTCACTTCCACTGGT 50.0 50.0 57.9 57.4 1355-1400 bp 1.0 75.9
rbcL-accD_p3 GCCGCTGCTTGTGAAATATG GGTTCTTCACTTCCACTGGT 50.0 50.0 58.2 57.4 1333-1378 bp 1.0 75.7
rbcL-accD_p4 TGAAGCTAGCAAATGGAGCC GGTTCTTCACTTCCACTGGT 50.0 50.0 58.2 57.4 1361-1406 bp 1.0 75.0
rbcL-accD_p5 TTTCGACCCAGTGGATAAGC GGTTCTTCACTTCCACTGGT 50.0 50.0 57.6 57.4 1295-1340 bp 1.0 73.9