Hotspot

trnM-CAU-atpE

LSC · 54895-55082 · 188 bp

Back to Neuwiedia

Pi 0.0529

MarkerSeek score 84.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 9
pi: 0.0529
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCTGGAGTCATTGGTTCAA	TAGGACACGAGTAGAGGCTG	50.0	55.0	58.1	58.0	249-263 bp	1.0	85.8
trnM-CAU-atpE_p2	GGCTGGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.1	58.0	262-276 bp	1.0	85.7
trnM-CAU-atpE_p3	GGCTGGAGTCATTGGTTCAA	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.1	58.0	272-286 bp	1.0	85.6
trnM-CAU-atpE_p4	GGCTGGAGTCATTGGTTCAA	GCTGAGGGTAAGAGACAAGC	50.0	55.0	58.1	58.0	299-313 bp	1.0	85.5
trnM-CAU-atpE_p5	GGCTGGAGTCATTGGTTCAA	TGAGGCGAATCTAGCTCTCA	50.0	50.0	58.1	57.9	276-290 bp	1.0	85.4