Hotspot

trnT-GGU-psbD

LSC · 34130-35219 · 1090 bp

Back to Neuwiedia

Pi 0.0421

MarkerSeek score 79.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 42
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AATTGAAGAAAACGGGCCCT	GGACCAGCCTACAAAAACGA	45.0	50.0	58.0	58.1	1221-1279 bp	1.0	85.7
trnT-GGU-psbD_p2	ATTGAAGAAAACGGGCCCTT	GGACCAGCCTACAAAAACGA	45.0	50.0	58.0	58.1	1220-1278 bp	1.0	85.7
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1192-1250 bp	1.0	85.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1195-1253 bp	1.0	85.2
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1211-1269 bp	1.0	85.0