Hotspot

trnE-UUC-trnT-GGU

LSC · 33515-34057 · 543 bp

Back to Neuwiedia

Pi 0.0714

MarkerSeek score 85.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 36
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGAGATGTCCTGGACCAC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	611-663 bp	1.0	86.9
trnE-UUC-trnT-GGU_p2	GAGAGATGTCCTGGACCACT	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	610-662 bp	1.0	86.9
trnE-UUC-trnT-GGU_p3	AGAGAGATGTCCTGGACCAC	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	608-660 bp	1.0	86.7
trnE-UUC-trnT-GGU_p4	GAGAGATGTCCTGGACCACT	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	607-659 bp	1.0	86.7
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	626-678 bp	1.0	86.5