Hotspot

trnC-GCA-petN

LSC · 29727-30382 · 656 bp

Back to Neuwiedia

Pi 0.105

MarkerSeek score 93.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 64
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 93.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.3	58.2	707-713 bp	1.0	87.8
trnC-GCA-petN_p2	CAGTTCGAATCTGGGTGTCG	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.6	58.2	706-712 bp	1.0	84.8
trnC-GCA-petN_p3	TAGGAAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.9	58.2	764-770 bp	1.0	82.3
trnC-GCA-petN_p4	CCAGTTCGAATCTGGGTGTC	GCCCAAGCGAGACTTACTAT	55.0	50.0	58.3	57.1	706-712 bp	1.0	81.9
trnC-GCA-petN_p5	TTAGGAAATTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.0	58.2	765-771 bp	1.0	81.8