Hotspot

rpoB-trnC-GCA

LSC · 28469-29655 · 1187 bp

Back to Neuwiedia

Pi 0.0232

MarkerSeek score 76.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1187
variable_sites: 26
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGCCAAGCCCTGATTAATGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.3	58.1	1326-1338 bp	1.0	82.4
rpoB-trnC-GCA_p2	CGCCAAGCCCTGATTAATGA	CGACACCCAGATTCGAACTG	50.0	55.0	58.3	58.6	1351-1363 bp	1.0	77.7
rpoB-trnC-GCA_p3	ATGAAATTCTTCCGCCAAGC	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.1	58.1	1338-1350 bp	1.0	77.4
rpoB-trnC-GCA_p4	CGCCAAGCCCTGATTAATGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.3	58.8	1364-1376 bp	1.0	76.7
rpoB-trnC-GCA_p5	GTGGACATTCCCTCATTTCCA	AAAGGATTTGCAGTCCCCTG	47.6	50.0	57.9	58.1	1262-1274 bp	1.0	76.4