Hotspot

matK-rps16

LSC · 3493-6130 · 2638 bp

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Pi 0.0899

MarkerSeek score 92.20

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 2638
variable_sites: 212
pi: 0.0899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 92.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TCCTGAAGGAGTAGCGGATA	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.0	58.2	2767-2796 bp	1.0	80.2
matK-rps16_p2	TCCTGAAGGAGTAGCGGATAT	GGTGCTCAACCTACAGGAAC	47.6	55.0	57.5	58.2	2767-2796 bp	1.0	76.4
matK-rps16_p3	TCCTGAAGGAGTAGCGGATA	GTGCTCAACCTACAGGAACT	50.0	50.0	57.0	57.2	2766-2795 bp	1.0	75.0
matK-rps16_p4	TCCTGAAGGAGTAGCGGAT	GGTGCTCAACCTACAGGAAC	52.6	55.0	57.1	58.2	2767-2796 bp	1.0	73.2
matK-rps16_p5	CCTGAAGGAGTAGCGGATAT	GGTGCTCAACCTACAGGAAC	50.0	55.0	55.9	58.2	2766-2795 bp	1.0	71.8