Pi
0.00476
MarkerSeek score
56.00
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | ACAGATGTCGTTTCTAGTCT | TCTTAACTGGGGCGGTAAGA | 40.0 | 50.0 | 53.5 | 58.1 | 1393 bp | 1.0 | 43.6 |
| ycf1_p2 | ACAGATGTCGTTTCTAGTCT | AACATTCTTAACTGGGGCGG | 40.0 | 50.0 | 53.5 | 58.2 | 1398 bp | 1.0 | 42.7 |
| ycf1_p3 | AACAGATGTCGTTTCTAGTCT | TCTTAACTGGGGCGGTAAGA | 38.1 | 50.0 | 54.4 | 58.1 | 1394 bp | 1.0 | 42.7 |
| ycf1_p4 | AACAGATGTCGTTTCTAGTCT | AACATTCTTAACTGGGGCGG | 38.1 | 50.0 | 54.4 | 58.2 | 1399 bp | 1.0 | 41.9 |
| ycf1_p5 | ACAGATGTCGTTTCTAGTCT | CTTAACTGGGGCGGTAAGAG | 40.0 | 55.0 | 53.5 | 57.7 | 1392 bp | 1.0 | 41.6 |
| ycf1_p1 | GCTATCTCTATTCGCGCGAT | CAAATGTGCATTCCCCTCTT | 50.0 | 45.0 | 57.8 | 56.3 | 223-229 bp | 1.0 | 68.6 |
| ycf1_p2 | CTATTCGCGCGATCTCCTTT | CAAATGTGCATTCCCCTCTT | 50.0 | 45.0 | 58.2 | 56.3 | 216-222 bp | 1.0 | 68.4 |
| ycf1_p3 | TATTCGCGCGATCTCCTTTC | CAAATGTGCATTCCCCTCTT | 50.0 | 45.0 | 58.2 | 56.3 | 215-221 bp | 1.0 | 68.3 |
| ycf1_p4 | TCCCAGGTCTCTGCTATCTC | CAAATGTGCATTCCCCTCTT | 55.0 | 45.0 | 57.6 | 56.3 | 235-241 bp | 1.0 | 66.9 |
| ycf1_p5 | CCCAGGTCTCTGCTATCTCT | CAAATGTGCATTCCCCTCTT | 55.0 | 45.0 | 57.6 | 56.3 | 234-240 bp | 1.0 | 66.8 |