Hotspot

ndhD

SSC · 116388-117890 · 1503 bp

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Pi 0.00233

MarkerSeek score 37.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 6
pi: 0.00233
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGTTGGGACCCAAAGGAAAC	TGGCCCTCTCTCATAAACCA	50.0	50.0	57.9	58.0	2453-2476 bp	1.0	79.8
ndhD_p2	GTTGGGACCCAAAGGAAACT	TGGCCCTCTCTCATAAACCA	50.0	50.0	57.9	58.0	2452-2475 bp	1.0	79.8
ndhD_p3	TTGTGGCGTCTATTGGCTTT	TGGCCCTCTCTCATAAACCA	45.0	50.0	58.1	58.0	2350-2373 bp	1.0	79.7
ndhD_p4	AATTGTGGCGTCTATTGGCT	TGGCCCTCTCTCATAAACCA	45.0	50.0	57.9	58.0	2352-2375 bp	1.0	79.5
ndhD_p5	ATTGTGGCGTCTATTGGCTT	TGGCCCTCTCTCATAAACCA	45.0	50.0	57.9	58.0	2351-2374 bp	1.0	79.5