Hotspot

ndhA

SSC · 120459-122642 · 2184 bp

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Pi 0.0013

MarkerSeek score 58.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2184
variable_sites: 5
pi: 0.0013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACTCAGTTACCATAGGAAACA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.6	57.8	2409-2423 bp	1.0	43.1
ndhA_p2	TCATGAACTCAGTTACCATAGGA	CCCACCGGGTTTTATCAACT	39.1	50.0	56.5	57.8	2412-2426 bp	1.0	42.2
ndhA_p3	TGTTTGAGACAAGTTGTGAA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2341-2355 bp	1.0	40.2
ndhA_p4	TTGTTTGAGACAAGTTGTGA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2342-2356 bp	1.0	40.2
ndhA_p5	TCATGAACTCAGTTACCATAGGAA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.1	57.8	2412-2426 bp	1.0	40.2