Hotspot

ccsA-ndhD

SSC · 116132-116387 · 256 bp

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Pi 0.00595
MarkerSeek score 61.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
256
variable_sites
3
pi
0.00595
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTGTGGCGTCTATTGGCTTT TCAGTTGACAAGGTCGAAGT 45.0 45.0 58.1 56.7 404-427 bp 1.0 72.0
ccsA-ndhD_p2 AATTGTGGCGTCTATTGGCT TCAGTTGACAAGGTCGAAGT 45.0 45.0 57.9 56.7 406-429 bp 1.0 71.7
ccsA-ndhD_p3 ATTGTGGCGTCTATTGGCTT TCAGTTGACAAGGTCGAAGT 45.0 45.0 57.9 56.7 405-428 bp 1.0 71.7
ccsA-ndhD_p4 TTGTGGCGTCTATTGGCTTT AGGTATTGGTATGTACCCCGA 45.0 47.6 58.1 57.7 441-464 bp 1.0 71.2
ccsA-ndhD_p5 ATTGTGGCGTCTATTGGCTT AGGTATTGGTATGTACCCCGA 45.0 47.6 57.9 57.7 442-465 bp 1.0 71.0