Hotspot

trnP(UGG)-psaJ

LSC · 68548-68948 · 401 bp

Back to Nestegis

Pi 0.00166

MarkerSeek score 60.30

Primer available yes

Label trnP(UGG)-psaJ

Feature Metadata

label_name: trnP(UGG)-psaJ
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 1
pi: 0.00166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP(UGG)-psaJ_p1	ACCCGTGACATTTTGTACCC	TAGTACTGGTGCAACGGAGA	50.0	50.0	58.1	58.1	489-506 bp	1.0	81.2
trnP(UGG)-psaJ_p2	CCCGTGACATTTTGTACCCA	TAGTACTGGTGCAACGGAGA	50.0	50.0	58.1	58.1	488-505 bp	1.0	81.2
trnP(UGG)-psaJ_p3	ACAGGATTTGAACCCGTGAC	TAGTACTGGTGCAACGGAGA	50.0	50.0	58.1	58.1	500-517 bp	1.0	81.1
trnP(UGG)-psaJ_p4	CAGGATTTGAACCCGTGACA	TAGTACTGGTGCAACGGAGA	50.0	50.0	58.1	58.1	499-516 bp	1.0	81.1
trnP(UGG)-psaJ_p5	TGACAGGATTTGAACCCGTG	TAGTACTGGTGCAACGGAGA	50.0	50.0	58.1	58.1	502-519 bp	1.0	81.1