Hotspot

psbE-petL

LSC · 66543-67725 · 1183 bp

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Pi 0.00873

MarkerSeek score 44.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 16
pi: 0.00873
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTTAAAGCCGCTAGTAAAA	50.0	35.0	57.9	52.5	1335 bp	1.0	41.1
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGTTAAAGCCGCTAGTAAAA	50.0	35.0	57.9	52.5	1348 bp	1.0	41.1
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGTTAAAGCCGCTAGTAAAAA	50.0	33.3	57.9	53.5	1335 bp	1.0	41.1
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGTTAAAGCCGCTAGTAAAAA	50.0	33.3	57.9	53.5	1348 bp	1.0	41.1
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTTAAAGCCGCTAGTAAAA	50.0	35.0	57.8	52.5	1258 bp	1.0	41.1