Hotspot

ycf4-cemA

LSC · 62048-62916 · 869 bp

Back to Nestegis

Pi 0.00403

MarkerSeek score 60.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 6
pi: 0.00403
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.0	58.1	1044-1056 bp	1.0	79.7
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1043-1055 bp	1.0	79.5
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1042-1054 bp	1.0	79.5
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	969-981 bp	1.0	79.0
ycf4-cemA_p5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	998-1010 bp	1.0	78.2