Hotspot

psbZ-trnG(GCC)

LSC · 37528-37817 · 290 bp

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Pi 0.0201

MarkerSeek score 48.50

Primer available yes

Label psbZ-trnG(GCC)

Feature Metadata

label_name: psbZ-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 290
variable_sites: 9
pi: 0.0201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG(GCC)_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	458-501 bp	1.0	79.8
psbZ-trnG(GCC)_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	459-502 bp	1.0	79.8
psbZ-trnG(GCC)_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	456-499 bp	1.0	79.4
psbZ-trnG(GCC)_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	457-500 bp	1.0	79.4
psbZ-trnG(GCC)_p5	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	55.0	50.0	57.5	58.1	393-436 bp	1.0	77.8