Hotspot

rps2-rpoC2

LSC · 17042-17249 · 208 bp

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Pi 0.0128

MarkerSeek score 61.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 4
pi: 0.0128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	377-386 bp	1.0	78.4
rps2-rpoC2_p2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	371-380 bp	1.0	78.4
rps2-rpoC2_p3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.8	57.8	374-383 bp	1.0	77.8
rps2-rpoC2_p4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	378-387 bp	1.0	77.3
rps2-rpoC2_p5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	55.0	50.0	58.3	57.8	375-384 bp	1.0	77.0