Hotspot

trnK-rps16

LSC · 4408-5313 · 906 bp

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Pi 0.00516

MarkerSeek score 50.20

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 8
pi: 0.00516
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAAGCCGAGTACTCTACCGT	ACCTAATCAAACGAAATTCAAT	50.0	27.3	58.2	52.4	957-965 bp	1.0	41.6
trnK-rps16_p2	AAGCCGAGTACTCTACCGTT	ACCTAATCAAACGAAATTCAAT	50.0	27.3	58.2	52.4	956-964 bp	1.0	41.6
trnK-rps16_p3	TCTAGCCGCACTTAAAAGCC	ACCTAATCAAACGAAATTCAAT	50.0	27.3	58.3	52.4	971-979 bp	1.0	41.6
trnK-rps16_p4	CCGCACTTAAAAGCCGAGTA	ACCTAATCAAACGAAATTCAAT	50.0	27.3	58.3	52.4	966-974 bp	1.0	41.6
trnK-rps16_p5	AAGCCGAGTACTCTACCGTT	ACCTAATCAAACGAAATTCAATT	50.0	26.1	58.2	53.2	956-964 bp	1.0	41.6