Hotspot

clpP1

LSC · 71910-74112 · 2203 bp

Back to Nervilia

Pi 0.095

MarkerSeek score 77.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2203
variable_sites: 207
pi: 0.095
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	GTTGGCATGGCGTGTCTATA	GATCGATCCCGTTCGTTACA	50.0	50.0	58.1	57.5	2913 bp	0.5	55.7
clpP1_p2	GTTGGCATGGCGTGTCTATA	CAATGGGAAGACTTTGGGGA	50.0	50.0	58.1	57.4	2989 bp	0.5	54.8
clpP1_p3	GTTGGCATGGCGTGTCTATA	CGATCGATCCCGTTCGTTAC	50.0	55.0	58.1	58.6	2914 bp	0.5	54.7
clpP1_p4	ATAGAGCGGGTTGGCTTAGA	GATCGATCCCGTTCGTTACA	50.0	50.0	58.2	57.5	2874 bp	0.5	54.5
clpP1_p5	TAGAGCGGGTTGGCTTAGAT	GATCGATCCCGTTCGTTACA	50.0	50.0	58.2	57.5	2873 bp	0.5	54.5