Hotspot

rrn5-rrn4.5

IRa · 133882-134094 · 213 bp

Back to Nervilia

Pi 0.0788

MarkerSeek score 84.40

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 213
variable_sites: 16
pi: 0.0788
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	318-328 bp	1.0	85.8
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	316-326 bp	1.0	85.8
rrn5-rrn4.5_p3	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	320-330 bp	1.0	85.7
rrn5-rrn4.5_p4	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	346-356 bp	1.0	85.3
rrn5-rrn4.5_p5	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	344-354 bp	1.0	85.3