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clpP1-psbB

LSC · 74113-74945 · 833 bp

Back to Nervilia

Pi 0.138

MarkerSeek score 83.00

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 105
pi: 0.138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	CATAACTCCAAATGCCCGGA	50.0	50.0	58.0	57.9	1189-1211 bp	1.0	91.3
clpP1-psbB_p2	CGCACTATACGTCAACCCAA	CAGATAGCTGCCAAGAAGCA	50.0	50.0	58.0	58.0	1247-1269 bp	1.0	91.2
clpP1-psbB_p3	CGCACTATACGTCAACCCAA	AGATAGCTGCCAAGAAGCAC	50.0	50.0	58.0	58.0	1246-1268 bp	1.0	91.2
clpP1-psbB_p4	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1414-1436 bp	1.0	90.7
clpP1-psbB_p5	CGCACTATACGTCAACCCAA	TAGACCCGCTAATATGCCCA	50.0	50.0	58.0	58.0	1551-1573 bp	1.0	89.8