Hotspot

accD-psaI

LSC · 60319-61140 · 822 bp

Back to Nervilia

Pi 0.0985

MarkerSeek score 83.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 77
pi: 0.0985
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATGCCTACATTGCGTTTGC	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.0	58.0	1127-1135 bp	1.0	89.6
accD-psaI_p2	AATGCCTACATTGCGTTTGC	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	1624-1635 bp	1.0	89.2
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	1190-1198 bp	1.0	89.1
accD-psaI_p4	AATGCCTACATTGCGTTTGC	TTGCTGAGACGGAAATACGG	45.0	50.0	58.0	58.0	1734-1745 bp	1.0	89.1
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TTGCTGAGACGGAAATACGG	55.0	50.0	58.0	58.0	1797-1808 bp	1.0	88.7