Hotspot

ndhC-trnV-UAC

LSC · 51272-52652 · 1381 bp

Back to Nervilia

Pi 0.156

MarkerSeek score 84.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1381
variable_sites: 199
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	36.4	55.0	56.1	58.1	1440-1628 bp	1.0	60.0
ndhC-trnV-UAC_p2	CATATTCGTGAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	38.1	55.0	54.6	58.1	1439-1627 bp	1.0	56.0
ndhC-trnV-UAC_p3	ATCATATTCGTGAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	34.8	55.0	56.6	58.1	1441-1629 bp	1.0	56.0
ndhC-trnV-UAC_p4	TCATATTCGTGAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	34.8	55.0	56.6	58.1	1440-1628 bp	1.0	56.0
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1425-1613 bp	1.0	55.4