Hotspot

psaA-pafI

LSC · 43286-44046 · 761 bp

Back to Nervilia

Pi 0.115

MarkerSeek score 83.50

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 80
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCATCAGCATGTAGGTTCCA	CTGAGCCGTATGAGGTAGGA	50.0	55.0	58.0	58.0	1103-1141 bp	1.0	89.9
psaA-pafI_p2	ATGCCACTCAGCCAAAGAAA	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1208-1246 bp	1.0	89.7
psaA-pafI_p3	CCCATTCCTCGAAAGACGTT	CTGAGCCGTATGAGGTAGGA	50.0	55.0	57.9	58.0	1024-1062 bp	1.0	89.7
psaA-pafI_p4	ATGCCACTCAGCCAAAGAAA	TGGGACGCAAAAAGAATCGA	45.0	45.0	58.0	58.1	1231-1269 bp	1.0	88.9
psaA-pafI_p5	CCAAGTCCTAGTAAACCCGC	CTGAGCCGTATGAGGTAGGA	55.0	55.0	58.0	58.0	1562-1600 bp	1.0	87.7