Hotspot

rpl2-trnH-GUG

IRa · 157175-157221 · 47 bp

Back to Nerine

Pi 0.0418
MarkerSeek score 66.80
Primer available yes
Label rpl2-trnH-GUG

Feature Metadata

label_name
rpl2-trnH-GUG
feature_type
igs
region
IRa
length_bp
47
variable_sites
5
pi
0.0418
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-trnH-GUG_p1 GTTATCCTGCGCTTGGAAGA TGGATCAAGGCAGTGGATTG 50.0 50.0 58.0 57.9 183 bp 1.0 80.9
rpl2-trnH-GUG_p2 GTTATCCTGCGCTTGGAAGA GGATCAAGGCAGTGGATTGT 50.0 50.0 58.0 57.9 182 bp 1.0 80.9
rpl2-trnH-GUG_p3 GTTATCCTGCGCTTGGAAGA GTGGATCAAGGCAGTGGATT 50.0 50.0 58.0 57.9 184 bp 1.0 80.9
rpl2-trnH-GUG_p4 GTTATCCTGCGCTTGGAAGA CGGATGTAGCCAAGTGGATC 50.0 55.0 58.0 58.1 197 bp 1.0 80.8
rpl2-trnH-GUG_p5 TATCCTGCGCTTGGAAGAAG CGGATGTAGCCAAGTGGATC 50.0 55.0 57.7 58.1 195 bp 1.0 78.5