Hotspot

ccsA-ndhD

SSC · 117842-118091 · 250 bp

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Pi 0.0385
MarkerSeek score 67.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
250
variable_sites
20
pi
0.0385
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GCCAAGTGCCATTTTTACCC TGGGCCACGAGAACTATTTG 50.0 50.0 57.9 57.9 1036-1067 bp 1.0 82.8
ccsA-ndhD_p2 GCCAAGTGCCATTTTTACCC GGGCCACGAGAACTATTTGT 50.0 50.0 57.9 57.9 1035-1066 bp 1.0 82.8
ccsA-ndhD_p3 GCCAAGTGCCATTTTTACCC TTGCATTGCCAGGAATGAGT 50.0 45.0 57.9 58.1 1250-1281 bp 1.0 82.7
ccsA-ndhD_p4 GCCAAGTGCCATTTTTACCC TGTCCCTGGTTGGTGATAGT 50.0 50.0 57.9 58.0 1578-1609 bp 1.0 82.6
ccsA-ndhD_p5 GCTAATGAGGCATGGGGATC TGTCCCTGGTTGGTGATAGT 55.0 50.0 58.2 58.0 1157-1188 bp 1.0 82.2