Hotspot

ndhF-rpl32

SSC · 114882-115830 · 949 bp

Back to Nerine

Pi 0.0773
MarkerSeek score 77.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
949
variable_sites
229
pi
0.0773
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACACTTGGAAAAGCCCACAT GGAAGTTCTTTGAGCCATGGA 45.0 47.6 57.9 58.2 1265-1289 bp 0.75 63.5
ndhF-rpl32_p2 GAAGAGGGAATTGTGCGGAT GGAAGTTCTTTGAGCCATGGA 50.0 47.6 58.0 58.2 1870-1894 bp 0.75 63.0
ndhF-rpl32_p3 TTTTTGACAAGCACTTGCCG GGAAGTTCTTTGAGCCATGGA 45.0 47.6 58.1 58.2 1662-1686 bp 0.75 62.7
ndhF-rpl32_p4 AGCAACTGCACCGACAAATA GGAAGTTCTTTGAGCCATGGA 45.0 47.6 58.1 58.2 1848-1872 bp 0.75 62.5
ndhF-rpl32_p5 TAGCAACTGCACCGACAAAT GGAAGTTCTTTGAGCCATGGA 45.0 47.6 58.1 58.2 1849-1873 bp 0.75 62.5