Hotspot

psbK-psbI

LSC · 7828-7992 · 165 bp

Back to Nerine

Pi 0.0456
MarkerSeek score 74.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
165
variable_sites
17
pi
0.0456
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACGAAGAGTTTGAGAGTAAGCA 50.0 40.9 57.9 57.4 218-226 bp 1.0 65.8
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT ACGAAGAGTTTGAGAGTAAGCA 45.0 40.9 58.3 57.4 215-223 bp 1.0 63.9
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT ACGAAGAGTTTGAGAGTAAGCA 45.0 40.9 58.3 57.4 216-224 bp 1.0 63.8
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT ACGAAGAGTTTGAGAGTAAGCA 45.0 40.9 58.3 57.4 217-225 bp 1.0 63.8
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG AACGAAGAGTTTGAGAGTAAGCA 50.0 39.1 57.9 58.1 219-227 bp 1.0 62.0