Hotspot

psbA-trnK-UUU

LSC · 1149-1386 · 238 bp

Back to Nerine

Pi 0.0342
MarkerSeek score 65.80
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
238
variable_sites
25
pi
0.0342
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 GCTTGTACTTTCGCGTCTCT ACTAGTTCCGGGTTCGAGTC 50.0 55.0 58.3 58.8 303-313 bp 1.0 78.8
psbA-trnK-UUU_p2 TAAAGACGGTTTTCGGTGCT ACTAGTTCCGGGTTCGAGTC 45.0 55.0 57.8 58.8 353-363 bp 1.0 78.1
psbA-trnK-UUU_p3 TAAAGACGGTTTTCGGTGCT GACTAGTTCCGGGTTCGAGT 45.0 55.0 57.8 58.8 354-364 bp 1.0 78.1
psbA-trnK-UUU_p4 AACACCGAACCATCCAATGT ACTAGTTCCGGGTTCGAGTC 45.0 55.0 57.7 58.8 372-382 bp 1.0 77.5
psbA-trnK-UUU_p5 AACACCGAACCATCCAATGT GACTAGTTCCGGGTTCGAGT 45.0 55.0 57.7 58.8 373-383 bp 1.0 77.4