Hotspot

psbC

LSC · 39716-41137 · 1422 bp

Back to Neptunia

Pi 0

MarkerSeek score 25.80

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TCTTTGCGCCTAGTTTTGGT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.0	58.0	2260 bp	1.0	80.0
psbC_p2	TGGTTCTTTGCGCCTAGTTT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.0	58.0	2264 bp	1.0	80.0
psbC_p3	TTGGTTCTTTGCGCCTAGTT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.0	58.0	2265 bp	1.0	80.0
psbC_p4	GATGGCGCAAATACATTCCG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	2084 bp	1.0	80.0
psbC_p5	ACCGGTTTATGGATGAGTGC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	1934 bp	1.0	80.0