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rps3-rps19

LSC · 91853-92644 · 792 bp

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Pi 0.0194

MarkerSeek score 65.20

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 14
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	TAAACTTCCGAGGACATGCG	50.0	50.0	57.5	58.0	808-879 bp	1.0	81.7
rps3-rps19_p2	TACCAAGTCTGAAACCGAGC	TCCGAGGACATGCGAAAAAT	50.0	45.0	57.5	57.9	802-873 bp	1.0	80.9
rps3-rps19_p3	GTCTGAAACCGAGCGGATTT	TAAACTTCCGAGGACATGCG	50.0	50.0	58.6	58.0	802-873 bp	1.0	80.8
rps3-rps19_p4	TACCAAGTCTGAAACCGAGC	TTCCGAGGACATGCGAAAAA	50.0	45.0	57.5	58.1	803-874 bp	1.0	80.8
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	CCGAGGACATGCGAAAAATG	50.0	50.0	57.5	57.8	801-872 bp	1.0	80.3