Hotspot

rpl33-rps18

LSC · 75639-76011 · 373 bp

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Pi 0.0161

MarkerSeek score 63.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 6
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	509-518 bp	1.0	77.8
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	510-519 bp	1.0	77.8
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	511-520 bp	1.0	76.0
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	483-492 bp	1.0	75.2
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	484-493 bp	1.0	75.2