Hotspot

psaJ-rpl33

LSC · 74872-75437 · 566 bp

Back to Neptunia

Pi 0.016

MarkerSeek score 53.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 9
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	TTGCAACAACAGGGACAGAA	50.0	45.0	58.1	57.9	1342-1343 bp	1.0	79.4
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	TTTGCAACAACAGGGACAGA	50.0	45.0	58.1	57.9	1343-1344 bp	1.0	79.4
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	TTGCAACAACAGGGACAGAA	50.0	45.0	58.1	57.9	1341-1342 bp	1.0	79.4
psaJ-rpl33_p4	CCCGTGACATTTTGTACCCA	TTTGCAACAACAGGGACAGA	50.0	45.0	58.1	57.9	1342-1343 bp	1.0	79.4
psaJ-rpl33_p5	ACAGGATTTGAACCCGTGAC	TTGCAACAACAGGGACAGAA	50.0	45.0	58.1	57.9	1353-1354 bp	1.0	79.3