Hotspot

accD-psaI

LSC · 66254-66921 · 668 bp

Back to Neptunia

Pi 0.0322

MarkerSeek score 71.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 21
pi: 0.0322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGCGGAATCTTTTAAAGGGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	811-815 bp	1.0	81.9
accD-psaI_p2	GCGGAATCTTTTAAAGGGCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	810-814 bp	1.0	81.9
accD-psaI_p3	ATTCAATCGTACCGCGGAAT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.8	58.0	823-827 bp	1.0	81.5
accD-psaI_p4	CGCGGAATCTTTTAAAGGGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	817-821 bp	1.0	81.3
accD-psaI_p5	GCGGAATCTTTTAAAGGGCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	816-820 bp	1.0	81.3