Hotspot

psbZ-trnG-GCC

LSC · 41996-42801 · 806 bp

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Pi 0.00671

MarkerSeek score 66.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 5
pi: 0.00671
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GACGGTTGGTCGAGTAACAA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.9	57.7	894-953 bp	1.0	80.7
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGACGGTTG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	58.4	57.7	906-965 bp	1.0	78.6
psbZ-trnG-GCC_p3	GACGGTTGGTCGAGTAACAA	TACATAAATAGAGGGCGCGG	50.0	50.0	57.9	57.3	937-996 bp	1.0	77.4
psbZ-trnG-GCC_p4	ATTTGCTTCTCCTGACGGTT	GCGTCTTCTCTTTGGCAAAG	45.0	50.0	57.4	57.7	907-966 bp	1.0	77.3
psbZ-trnG-GCC_p5	ACGGTTGGTCGAGTAACAAA	GCGTCTTCTCTTTGGCAAAG	45.0	50.0	57.4	57.7	893-952 bp	1.0	77.1