Hotspot

psbC-trnS-UGA

LSC · 41138-41379 · 242 bp

Back to Neptunia

Pi 0.0455

MarkerSeek score 78.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 11
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CAGCGGGATTTGAAAAAGGC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	402 bp	1.0	79.7
psbC-trnS-UGA_p2	GCAGCGGGATTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	403 bp	1.0	79.7
psbC-trnS-UGA_p3	CAGCGGGATTTGAAAAAGGC	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.3	58.1	394 bp	1.0	79.5
psbC-trnS-UGA_p4	CAGCGGGATTTGAAAAAGGC	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.3	58.1	395 bp	1.0	79.5
psbC-trnS-UGA_p5	GCAGCGGGATTTGAAAAAGG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.3	58.1	395 bp	1.0	79.5