Hotspot

trnC-GCA-petN

LSC · 31873-33287 · 1415 bp

Back to Neptunia

Pi 0.0144

MarkerSeek score 68.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1415
variable_sites: 20
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1457-1471 bp	1.0	80.8
trnC-GCA-petN_p2	GGTATCATTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1510-1524 bp	1.0	80.2
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1509-1523 bp	1.0	80.2
trnC-GCA-petN_p4	TGGTATCATTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.2	58.2	1511-1525 bp	1.0	79.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1518-1532 bp	1.0	79.1