Hotspot

rps16-trnQ-UUG

LSC · 7113-7930 · 818 bp

Back to Neptunia

Pi 0.0214
MarkerSeek score 68.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
818
variable_sites
16
pi
0.0214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GTCGAATAGAAATGGGGCGT 50.0 50.0 58.0 58.1 886-958 bp 1.0 84.9
rps16-trnQ-UUG_p2 GTCTTTCAAGTCGCACGTTG TCGAATAGAAATGGGGCGTG 50.0 50.0 58.0 58.1 885-957 bp 1.0 84.9
rps16-trnQ-UUG_p3 GTCTTTCAAGTCGCACGTTG TGTCGAATAGAAATGGGGCG 50.0 50.0 58.0 58.1 887-959 bp 1.0 84.9
rps16-trnQ-UUG_p4 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 826-898 bp 1.0 84.8
rps16-trnQ-UUG_p5 ATGTCTTTCAAGTCGCACGT GTCGAATAGAAATGGGGCGT 45.0 50.0 58.1 58.1 888-960 bp 1.0 83.9