Hotspot

trnK-UUU-rps16

LSC · 4424-5916 · 1493 bp

Back to Neptunia

Pi 0.0175
MarkerSeek score 72.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1493
variable_sites
25
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1670-1706 bp 1.0 83.7
trnK-UUU-rps16_p2 TGGAGTCGCACTTAAAAGCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.5 58.0 1624-1660 bp 1.0 80.6
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC AAAAGGCGCTCAACCTACAG 55.0 50.0 57.9 58.5 1669-1705 bp 1.0 80.1
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG AAAAAGGCGCTCAACCTACA 50.0 45.0 58.6 58.0 1621-1657 bp 1.0 79.9
trnK-UUU-rps16_p5 GAGTCGCACTTAAAAGCCGA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.6 58.0 1622-1658 bp 1.0 79.9