Hotspot

trnH-GUG-psbA

LSC · 79-466 · 388 bp

Back to Neptunia

Pi 0.018
MarkerSeek score 64.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
388
variable_sites
7
pi
0.018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 457-460 bp 1.0 79.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 471-474 bp 1.0 79.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 470-473 bp 1.0 79.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 472-475 bp 1.0 79.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCGAGCTAACCTTGGTATGG 55.0 55.0 58.1 57.8 506-509 bp 1.0 78.1