Hotspot

psaC-ndhE

SSC · 113006-113293 · 288 bp

Back to Nephrolepis

Pi 0.118

MarkerSeek score 78.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 288
variable_sites: 32
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GATTTGCTTTGCACCCATCC	AGGGCACTTATGAGTCTCGA	50.0	50.0	58.0	57.9	612-622 bp	1.0	86.9
psaC-ndhE_p2	CTGCGAGTAGTTTCAGACCC	AGGGCACTTATGAGTCTCGA	55.0	50.0	58.0	57.9	737 bp	0.5	58.8
psaC-ndhE_p3	CGGACCTGACAATCCTGAAG	AGGGCACTTATGAGTCTCGA	55.0	50.0	58.0	57.9	832 bp	0.5	58.7
psaC-ndhE_p4	AATACATCGGTGGGACATGC	AGGGCACTTATGAGTCTCGA	50.0	50.0	58.0	57.9	587 bp	0.5	58.6
psaC-ndhE_p5	CAGACCCTAGGTAGACACGT	AGGGCACTTATGAGTCTCGA	55.0	50.0	57.9	57.9	724 bp	0.5	57.8