Hotspot

rpl32-trnP-GGG

SSC · 108488-109081 · 594 bp

Back to Nephrolepis

Pi 0.113
MarkerSeek score 83.10
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
SSC
length_bp
594
variable_sites
64
pi
0.113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 TGGTTGAATGATGCAGCTGT CTCTGGCACTGCTTCCTAAG 45.0 55.0 58.1 58.0 1557-1567 bp 1.0 89.7
rpl32-trnP-GGG_p2 TGGTTGAATGATGCAGCTGT CTGGCACTGCTTCCTAAGAG 45.0 55.0 58.1 58.0 1555-1565 bp 1.0 89.7
rpl32-trnP-GGG_p3 ATTTTACGATGCTCGGCACT CTCTGGCACTGCTTCCTAAG 45.0 55.0 58.0 58.0 1685 bp 0.5 59.6
rpl32-trnP-GGG_p4 ATTTTACGATGCTCGGCACT CTGGCACTGCTTCCTAAGAG 45.0 55.0 58.0 58.0 1683 bp 0.5 59.6
rpl32-trnP-GGG_p5 ATTTTACGATGCTCGGCACT TACACCAGTTTAGGAGGGCA 45.0 50.0 58.0 58.0 1589 bp 0.5 59.5