Hotspot

petG-trnW-CCA

LSC · 62337-62604 · 268 bp

Back to Nephrolepis

Pi 0.116

MarkerSeek score 80.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 268
variable_sites: 30
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CCACAATATCGACGTGGTGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.7	57.9	343-346 bp	1.0	86.0
petG-trnW-CCA_p2	ATCGGGAATTGTGTTGGGTT	CAAAACCCGATGTCGTAGGT	45.0	50.0	57.7	57.9	404-407 bp	1.0	84.6
petG-trnW-CCA_p3	TGTGTTGGGTTCGATTCCAA	CAAAACCCGATGTCGTAGGT	45.0	50.0	57.6	57.9	395-398 bp	1.0	84.4
petG-trnW-CCA_p4	TTGTGTTGGGTTCGATTCCA	CAAAACCCGATGTCGTAGGT	45.0	50.0	57.6	57.9	396-399 bp	1.0	84.3
petG-trnW-CCA_p5	GGGAATTGTGTTGGGTTCGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.4	57.9	401-404 bp	1.0	83.9