Hotspot

ndhC-trnV-UAC

LSC · 48195-48674 · 480 bp

Back to Nephrolepis

Pi 0.0861
MarkerSeek score 78.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
480
variable_sites
41
pi
0.0861
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGAGTCATATTGGTGGAGCG TGCATGACCGAGAGTGTCTA 55.0 50.0 58.1 58.2 549-550 bp 1.0 84.2
ndhC-trnV-UAC_p2 GGAGTCATATTGGTGGAGCG GCATGACCGAGAGTGTCTAC 55.0 55.0 58.1 57.8 548-549 bp 1.0 84.0
ndhC-trnV-UAC_p3 TATTGGTGGAGCGAAAACGT TGCATGACCGAGAGTGTCTA 45.0 50.0 57.8 58.2 542-543 bp 1.0 83.9
ndhC-trnV-UAC_p4 TATTGGTGGAGCGAAAACGT GCATGACCGAGAGTGTCTAC 45.0 55.0 57.8 57.8 541-542 bp 1.0 83.7
ndhC-trnV-UAC_p5 GGAGTCATATTGGTGGAGCG CATGACCGAGAGTGTCTACG 55.0 55.0 58.1 57.5 547-548 bp 1.0 81.9