Hotspot

psbD-trnT-GGU

LSC · 33910-35254 · 1345 bp

Back to Nephrolepis

Pi 0.0739
MarkerSeek score 79.70
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1345
variable_sites
97
pi
0.0739
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 TTTTCCGATGGCAATGGTCA TTGATCTGAGGCCCCCTTAA 45.0 50.0 58.1 58.0 1433-1448 bp 1.0 86.3
psbD-trnT-GGU_p2 TTTTCCGATGGCAATGGTCA TTTGATCTGAGGCCCCCTTA 45.0 50.0 58.1 58.0 1434-1449 bp 1.0 86.3
psbD-trnT-GGU_p3 TTTCCGATGGCAATGGTCAT TTGATCTGAGGCCCCCTTAA 45.0 50.0 57.9 58.0 1432-1447 bp 1.0 85.9
psbD-trnT-GGU_p4 TTTCCGATGGCAATGGTCAT TTTGATCTGAGGCCCCCTTA 45.0 50.0 57.9 58.0 1433-1448 bp 1.0 85.9
psbD-trnT-GGU_p5 TTTTCCGATGGCAATGGTCA ATTTTTGATCTGAGGCCCCC 45.0 50.0 58.1 57.8 1437-1452 bp 1.0 85.2