Hotspot

trnC-GCA-trnG-GCC

LSC · 29756-30196 · 441 bp

Back to Nephrolepis

Pi 0.131

MarkerSeek score 85.20

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 53
pi: 0.131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	501-522 bp	1.0	81.7
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGGTTCGATTCCCGCTATC	50.0	57.9	57.9	57.8	503-524 bp	1.0	81.5
trnC-GCA-trnG-GCC_p3	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	502-523 bp	1.0	81.5
trnC-GCA-trnG-GCC_p4	TCCGTCTTACCGCTTGACTA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.2	58.2	489-510 bp	1.0	81.1
trnC-GCA-trnG-GCC_p5	TCCGTCTTACCGCTTGACTA	GGTTCGATTCCCGCTATCC	50.0	57.9	58.2	57.8	490-511 bp	1.0	80.9