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psbM-petN

LSC · 28001-29148 · 1148 bp

Back to Nephrolepis

Pi 0.0971

MarkerSeek score 84.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 109
pi: 0.0971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	CTTTACATTCAAACGGCCGC	TTGTAGTTTGGGGACGAAGC	50.0	50.0	58.4	58.1	1195-1210 bp	1.0	84.9
psbM-petN_p2	TCTGATCCCGACAGCTTTTC	TTGTAGTTTGGGGACGAAGC	50.0	50.0	57.6	58.1	1223-1238 bp	1.0	84.7
psbM-petN_p3	CTGATCCCGACAGCTTTTCT	TTGTAGTTTGGGGACGAAGC	50.0	50.0	57.6	58.1	1222-1237 bp	1.0	84.7
psbM-petN_p4	TCTGATCCCGACAGCTTTTC	CACTTGTAGTTTGGGGACGA	50.0	50.0	57.6	57.5	1226-1241 bp	1.0	81.3
psbM-petN_p5	TCTGATCCCGACAGCTTTTC	TCACTTGTAGTTTGGGGACG	50.0	50.0	57.6	57.5	1227-1242 bp	1.0	81.3