Hotspot

rpoB-trnD-GUC

LSC · 26544-27171 · 628 bp

Back to Nephrolepis

Pi 0.0805

MarkerSeek score 83.50

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 50
pi: 0.0805
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	TGCAAGTTCTGGCACTACG	GAGTTCATCGGGATTGACGG	52.6	55.0	58.1	58.4	734-741 bp	1.0	74.1
rpoB-trnD-GUC_p2	TGCAAGTTCTGGCACTACG	GGAGTTCATCGGGATTGACG	52.6	55.0	58.1	58.4	735-742 bp	1.0	74.1
rpoB-trnD-GUC_p3	GAATTTGTGCAAGTTCTGGCA	GAGTTCATCGGGATTGACGG	42.9	55.0	57.9	58.4	741-748 bp	1.0	73.9
rpoB-trnD-GUC_p4	GAATTTGTGCAAGTTCTGGCA	GGAGTTCATCGGGATTGACG	42.9	55.0	57.9	58.4	742-749 bp	1.0	73.9
rpoB-trnD-GUC_p5	TGAATTTGTGCAAGTTCTGGC	GAGTTCATCGGGATTGACGG	42.9	55.0	57.9	58.4	742-749 bp	1.0	73.9