Hotspot

atpH-atpI

LSC · 13559-14264 · 706 bp

Back to Nephrolepis

Pi 0.0799

MarkerSeek score 78.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 55
pi: 0.0799
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TTCTGGCTGTCTCGCAATAC	CCACGGAAGGCCATCATTAA	50.0	50.0	58.0	57.9	858-884 bp	1.0	86.4
atpH-atpI_p2	TTCTGGCTGTCTCGCAATAC	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.0	58.0	989-1015 bp	1.0	86.2
atpH-atpI_p3	GCTAGGGCTACAACCAATCC	GTTGGTAGTAGCTGTTCCCG	55.0	55.0	58.0	58.0	1075-1101 bp	1.0	85.8
atpH-atpI_p4	AAACAATATCTGGTCCGGCC	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	57.9	58.0	1437 bp	0.5	59.5
atpH-atpI_p5	AGTGGGGACTCGATTGGTTA	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.1	58.0	1240 bp	0.5	59.5